Giesse Technology plasma sanitation | plasma treatment | plasma surface treatment | Morphological Analysis of Surface

Morphological Analysis of Surface

GIESSE TECHNOLOGY has introduced a dedicated surface treatment process called HSS (High Speed Surface) for its dental implants. The new treatment, resulting from the application of a sandblasting treatment followed by an acid etching process, allows to obtain a roughness of the open porosity surface, particularly indicated to favor the osseointegration of the dental implant.

Morphological  Characterization

In collaboration with the University of Trieste, a morphological characterization of the surface of dental implants treated with HSS was perfomed. The analyses carried out at the University of Trieste, by means of a three- dimensional profilometer, showed that the roughness of the HSS surface was significantly greater than the one obtained with the sole sandblasting process, and equivalent to the one described in the literature for dental implants subjected to Sandblasted Large-grid and Acid-etched, SLA. In addition, the remarkable homogeneity of the treatmenton the entire surface was highlighted.

Absence of Superficial Contamination

The chemical analysis of the surface of the HSS treated implant highlights the absence of contaminating elements, and in particular, of the alumina used for sandblasting (present on the surfaces treated only with sandblasting), and the presence of titanium oxide phosphate hydrate, itroduced through HSS treatment. This type of oxide promotes cell adhesion by osteoblasts, whit a consequent increase in the quality of osseointegration.

High Wettability

HSS treatment also has a positive influence on the wettability of the implant surface.

In the following images, it is possible to notice the greater wettability of the surface of a HSS-treated implant compared to the only sandblasted surface.

The contact angle of the cell culture serum passes from 55°C for the sandblasted surface to only 38°C for the HSS-treated surface. Thhis parameter is an index of the speed of protein absorption which will then influence the cell adhesion and consequently the osseointegration.

Biological Tests of Cellular Proliferation

At the University of Trieste, the surface of GiEsse Technology dental implants treated with HSS was subjected to cell proliferation analysis to evaluate its biological response.

Cell proliferation on the surface of the HSS implant was extremely high in the first nine days of cell application compared to an identical implant treated only with sandblasting, resulting in a faste r cell differentiation. After the ninth day, growth tends are similar. It is important to pay attention to the fact that the cell proliferation of the HSS system is similar to that of the cells present on the control. Below, we see images obtained through the epifluorescence microscope. Cells show a typical morphology of fibroblasts derived from human osteosarcoma (MG-63); we observe a massive concentration of cells highly adherent to the substrate (presence of many filopodia), organised on several layers and a massive amount of extracellular matrix.

Epifluorescence microscope images.

Cellular cytoskeleton, labelled with FITC - phalloidin (green) and the nucleus labelled with DAPI (blue).